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The model of deficit accumulation also needs to be evaluated with cellular and animal data.
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To identify the phenotype of cells seeded on the rhBMP-2-conjugated scaffold, cellular activity was evaluated with scanning electron microscopy and with viability, histological, and immunohistochemical testing.
Statistical analyses of biochemical and cellular data were evaluated with GraphPad Prism 5 employing t‐tests.
At the end of the treatment, cellular vitality was evaluated with the Trypan blue dye exclusion test, and chondrocytes were cultured in 24-well plate in DMEM supplemented with 10% FCS for 48 hours prior to flow-cytometry.
Cellular ROS levels were evaluated with the cell permeant probe carboxy-H2DCFDA (5- and-6 -carboxy-2′,7′-dichlorodihydrofluorescein 5- and-6 -carboxy-2′,7′-dichlorodihydrofluorescein 5- and-6 -carboxy-2′,7′-dichlorodihydrofluorescein 5- and-6 -carboxy-2′,7′-dichlorodihydrofluorescein 5- and-6 -carboxy-2′,7′-dichlorodihydrofluorescein 5- and-6 -carboxy-2′,7′-dichlorodihydrofluorescein
Cellular ROS levels were evaluated with the cell permeant probe CM-H2DCFDA (5- and-6 -chloromethyl-2'7'-dichlorodihydrofluorescein 5- and-6 -chloromethyl-2'7'-dichlorodihydrofluorescein 5- and-6 -chloromethyl-2'7'-dichlorodihydrofluorescein 5- and-6 -chloromethyl-2'7'-dichlorodihydrofluorescein 5- and-6 -chloromethyl-2'7'-dichlorodihydrofluorescein 5- and-6 -chloromethyl-2'7'-dichlorodihydrofluorescein
The cellular orientation inside implanted scaffolds was evaluated with immunofluorescence.
The in vitro cellular uptake of SWCNT- KWKG 7- PEG 12/pDNA complex prepared with fluoreSWCNT- KWKG 7- PEG 12/pDNAaluated with fluorescomplexcroscopreparedrvation and flowithtometry.
Pedigree relationships were evaluated with KINGROUP v2.0.8.
Heterogeneity was evaluated with Chi-square test.
Period differences were evaluated with t-test.
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