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Assay performance of a set of selected external RNA transcripts or pools with the same or different concentrations will be evaluated by microarray and QRT-PCR.
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Eleven HS proteoglycans has been identified so far – syndecan 1 4, glypican 1 6, CD44 isoforms containing the alternatively spliced exon v3 – and their gene expression can be evaluated by microarrays[ 68] (see Additional file 3).
Following incubation with the extract, total RNA was purified from the cells and mRNA expression levels were evaluated by microarray analysis using an Affymetrix platform.
The transcriptional response was evaluated by microarray at 30 minutes post exposure to 30 µM H2O2 and the proteome was evaluated by 2D-DIGE at both 30 and 105 minutes post exposure to 30 µM H2O2.
Global mammary gland expression profiles were evaluated by microarray analysis.
The differential expression of crucial genes in Down progenitor cells was evaluated by microarray analysis.
Mammary gland differentiation was examined by immunohistochemistry. Gene expression was evaluated by microarray, qRT-PCR and quantitative western blotting assays.
The gene expression of MCF-7 cells treated with Si-Wu-Tang was evaluated by microarray, and the potential targets of each herb were identified using the TCM Integrative Database.
Importantly, in the same study, and despite marked differences in protein recovery yield of sample homogenates processed directly in TRIzol® or by AFD followed by TRIzol®, these samples were evaluated by microarray and 2DE analyses.
Specificity of BAY 87-2243 for the suppression of HIF-1-mediated gene transcription on a genome-wide scale was evaluated by microarray hybridizations using Affymetrix whole genome 133Av2 arrays.
Cells were stimulated for 6 and 24 hours (h) with LPS (TLR4), CLO97 (TLR7/8), poly I C complexed with Lyovec (MDA-5/RIGI), or 5′-pppRNA complexed with Lyovec (RIG-I) (see Experimental procedures), and the scope of PRR responses to innate agonist stimulation was evaluated by microarray analysis using Illumina BeadChips (see Experimental procedures).
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