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The QALY value will be estimated using straight line interpolation between data points.

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Because the path of the contact front is assumed to be linear in the PAVA method, the best-fit axis of the transect orientation through sampled individuals was estimated using a one-dimensional cline fitting with a straight centerline.

In the case of the conventional CT, the interior is estimated using a mathematical inverse transform based on an assumption that paths are straight and parallel.

1/S' was estimated using BlastPhen.

Nucleotide diversity was estimated using DnaSp 4.10 [38].

Confidence intervals were estimated using normal approximation.

RNA concentration was estimated using nanodrop spectrophotometer.

Divergence times were estimated using Multidivtime [52] [54].

Protein concentration was estimated using Coomassie Plus Protein Reagent.

Effects were estimated using conditional logistic regression.

Statistical power was estimated using 1000 simulations.

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