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However, it is always questionable whether accurate motion fields can be estimated using real data.
If cost-guidelines are not available, costs will be estimated using real prices or population-based estimates if available in the literature.
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The thermal inertia of the recuperator, which plays a critical role in the transient behavior of the engine, was estimated using real shut-down data.
If no guideline costs existed, cost prizes were estimated using real costs and tariffs.
The total electron content (TEC) along a vertical ray path through the ionosphere was estimated using real-time spatial maps of the peak electron density in the F2 region.
The mRNA expression level of the Ang II receptors (AT1 and AT2), the relaxin receptor (LGR7), and the inducible nitric oxide synthase (iNOS) were estimated using real-time RT-PCR and a GeneAmp 5700 System (Applied Biosystems, USA).
The enrichment of DNA on tested genomic regions was estimated using real-time PCR (qPCR).
First of all, the relative expression of GNAO1 in HCC and adjacent non-cancerous tissues was estimated using real-time qPCR and IHC.
Expression of mecA, an antibiotic-resistant gene of MRSA, as well that of sea, agrA, and sarA and virulence-factor genes was estimated using real-time PCR in sub-MIC.
The quantity of the EHV-1 DNA in all samples was estimated using real-time PCR (RT-PCR, qPCR) with fluorescent TaqMan probes (150 nM), complementary to a sequence within the amplified products.
Changes in gene copy number and mRNA/miR expression were estimated using real-time polymerase chain reaction assays in two mammalian cell lines, MCF-7 and HeLa, and in a set of sporadic breast cancer tissues.
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