However, for actual experiments or commercial release, copy number has to be established without error, and for such applications real-time PCR must be able to precisely distinguish plants with one, two, or three transgenes.
The reason for the increased rate of coating formation could not be established without further investigation, although several possible explanations have been proposed.
The 17- and 6-year-old systems were abandoned Terminalia ivorensis plantations which had been established without any agrochemical use and had not received any management in the interim (Norgrove et al. 2003a, 2003b).
Newly contrived constitutional rights have been established without any apparent concern for the empirical process that goes with legislative reform.
The Galapagos Islands are a prime example of a place where fishery management policies have been established without first understanding the behavior of fishermen.
The newspaper was reporting the "white terror" in Berlin, referring to the Reinhardt regiment "which it has been established without a shadow of doubt today is responsible for the murder of Karl Liebknecht and Rosa Luxembourg [sic]" and quoting an eye-witness account of how they had been "done to death by soldiers' patrols with the butt-ends of their rifles and afterwards shot".
Bell, a former communications adviser to Margaret Thatcher, is furious about the methods used to secure the story and has filed a complaint with the Press Complaints Commission which claims that there was no proof of impropriety, and that the same information could have been established without undercover reporting.
(b) Entering designated entrance fee areas or using specialized sites, facilities, equipment or services, or participating in group activities, recreation events, or other specialized recreation uses for which recreation fees have been established without paying the required fees and possessing the applicable permits is prohibited.
