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In early in vivo studies, PHA-408 demonstrated efficacy at high doses; however, the correlation between PHA-408 exposure and efficacy could not be established using standard dosing paradigms for the rat disease models.
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An abundance of such mind-brain linkages have been established using standard neuropsychological and brain-imaging procedures.
The clinical diagnosis of CD was established using standard clinical, endoscopic, and histopathological criteria according to the Montreal classification [45].
The clinical diagnosis of CD was established using standard clinical and endoscopic criteria according to the Vienna classification [34] and confirmed by histological examination of gut tissue.
A calibration curve was established using standard dilutions of mouse anti-MOG IgG and individual values of serum anti-MOG IgGs were then calculated and expressed as ng/ml.
Transgenic lines were established using standard methods at the Indiana University Transgenic and Knock-out Mouse Core FacIndiana University Transgenic and Knock-out
All measurements were done using a three-colour setup, which was established using standard compensation procedures.
9 Skin punch biopsies were obtained and primary fibroblast cell lines were established using standard procedures.
The stably engineered pMSCV-miR-1269 pMSCV-miR-1269 pMSCV-miR-1269ng standard methods [ 14].
The presence of sepsis, severe sepsis, and septic shock was established using standard criteria [ 14].
Ten 20 ×20 m plots were established using standard protocols for vegetation community analyses [ 31, 32, 48].
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