A synchronized life cycle of interconnected energy systems is established through direct interconnections among the processes of different energy systems, and all interconnecting systems are bounded within the same timeframe.
The genotypic and allelic frequencies for the seven polymorphisms were established through direct counting.
The completeness and consistency of data was established through direct and daily supervision by the supervisor and principal investigator.
Since many of the MYC target genes are TCFs themselves, 29 waves of time-dependent transcription are established through direct and indirect target gene regulation.
Pedigrees for the population were established through direct field observation in combination with molecular parentage verification using microsatellite markers [ 40, 93, 97].
To further determine if BMI1 expression was replicated, we examined BMI1 mRNA expression in a panel of 8 patient-tumor derived pGBM xenograft models (Table 1), which were established through direct implantation of surgical specimens to the matched locations in mouse brains and strictly subtransplanted in vivo the brains of SCID mice [ 45, 50].
This demonstrates that hierarchical suppression can be established through either variable sensory gain or direct unidirectional inhibition, in conjunction with winner-take-all selection of the module with the highest activation level.
A definitive role of TRPA1 as a direct mechanically-gated ion channel could potentially be established through mechanical stimulation of heterologous cells expressing TRPA1.
Although not included in the design or the scope of this study, direct control of target gene expression exerted by miRNAs may be established through functional studies that evaluate correlations with mRNA and protein levels [ 35, 36].
During this process of organellogenesis, a nucleus-to-organelle flow of biogenetic information was established through the progressive ability to direct nuclear-encoded proteins synthesized in the cytosol to specific subcellular locations within the endosymbionts [ 1, 2, 5].
