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Historic districts cannot be established overnight.
This is a good first step.Obviously, secure property rights cannot be established overnight.
Hardly anyone I talked to believes that democracy can be established overnight.
No one I talked to believes that democracy can be established overnight (well, almost, 18-year-olds think it's possible, of course).
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Biofilms of S. aureus ATCC 29213 and three clinical isolates were established overnight and treated with lysostaphin or Conflikt® for 20 h.
On both study days, identical and stable glycemia (blood glucose target 6.0 mmol/l) and insulinemia (basal intravenous [IV] infusion of regular human insulin [RHI] 0.2 mU · kg−1 · min−1) were established overnight by automated glucose clamps.
Vitamin C stimulated the formation of PAEC into tube networks, which were established following overnight incubation.
The B. anthracis biofilms were established from overnight 5 ml BHI cultures (with 0.2% glucose) that were washed twice in PBS, resuspended in 5 ml of PBS, and diluted 1∶1000 into 10 ml of BHI.
For this reason, the conditions of 0.4 M of IPTG and 18°C (overnight) were established as the best conditions of TcPR-4b induction.
Four replicate overnight cultures were established for each of the four treatments, PF, P2, P4, and P2&4 making a total of 16 cultures.
For each strain, forty-eight 100 μl cultures and forty-eight 200 μl cultures of a 1 10,000 dilution of a saturated overnight culture were established in a 96-well plate.
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