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Such a council should be established in parallel to the ongoing prevention trial.
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Clones expressing human APOBEC3G cDNA or an empty vector control were established in parallel (Figure 2A).
Cultures of HIV RNA-electroporated mature DC, and eGFP-RNA control DC were established in parallel with CFSE-labeled PBMC at a 1∶10 ratio, 1 million total cells/mL in 5% Human AB serum for 6 days at 37°C, 5% CO2.
Transfections omitting the DNA constructs were established in parallel as negative controls.
Appropriate cell, Lipofectamine 2000 and negative siRNA controls were established in parallel.
For all experiments, no-template controls were established in parallel with diluent added without cfDNA extract (Table S5).
In this study, for the first time, two screening methods were established in parallel for the isolation of novel XI-encoding genes from a soil metagenomic library.
However, breast diagnostic centres were established in parallel with the Norwegian mammography screening programme and resulted in centralisation of care for women with breast cancer.
By mixing equal amount of RNA of the four stages, RNA pools from both QS and EG were established in parallel.
Moreover, to quantify the quality of breast cancer services and improvements in the quality of breast cancer care, a Germany-wide voluntary benchmarking programme was established in parallel with the certification programme.
ASM cell monoculture control cell counts were established in parallel with mast cell co-cultures using the Kimura staining, which readily differentiates red metachromatic mast cells from unlabelled ASM cells.
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