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As mentioned above, human ES cells can be established from pre-implantation embryos under conditions used to establish mouse post-implantation (not pre-implantation) pluripotent cell lines [ 2,3].
Accordingly, pluripotent cell lines can be established from the pre-implantation or post-implantation mouse embryo as embryonic stem (ES) or epiblast stem (EpiSC) cell lines, respectively.
The primary cell culture L2635 was established from a pre-treatment biopsy of osteosarcoma patient 398 as described previously and maintained in RPMI 1640 with 20% FCS and 1% P/S [ 20].
The MTX resistant cell line, M316, was established from a relapsed pre-B ALL patient at MSKCC.
Geomorphic landscape development in the pre-Holocene ancestral Columbia River Valley (1 5 km width) in the Portland forearc basin (~ 50 km length) is established from depositional sequences, which pre-date and post-date the glacial Lake Missoula floods.
MBP (myelin basic protein -specific T-cell clones were established from lymprotein -specificons of pre-immunized Lewis rats.
The coding framework used was established from the responses and was not pre-determined.
Passage 1 3 fibroblast cultures were established from surplus amniocentesis samples used in pre-natal diagnosis.
The concentration effect relationship for MP with TOCP pre-treatment was established from exposures at 238, 314 and 412 ppm MF.
Liquid cultures were established from single colonies.
Therefore, separate quartiles were established for pre- and postmenopausal women using the distributions from the control population.
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