This concentration was established by adding algae from a start culture.
Thirdly, the 3D structural design of each module is established by adding side walls, cover plates, stiffening plates, and other components based on the radial building.
The level of performance on each task was established by adding the time to completion of a task with penalty points, consequently rewarding precision and speed.
Else, an adaptive refinement of the design of experiments is established by adding optimally selected new support points for informing the metamodel development, and the next SAO cycle remains in the current sub-region.
BRCE system is established by adding hydrophobic l-iso-butyl tartrate in organic phase and hydrophilic β-cyclodextrin (β-CD) derivative in aqueous phase, which preferentially recognize S-H2A and R-H2A, respectively.
Among the 21643 nuclear genes present on the CATMA array, 3312 were deregulated in at least one condition in lhp1, whereas 1008 were deregulated in lif2 (the lists of genes were established by adding deregulated genes in the different biological materials and by removing duplicates) (Fig. 6A).
The pH 6.0 ± 0.1 condition was established by adding 5 mM MES buffer (2-morpholinoethansulfonic acid) to the medium.
In some trials, dietary fat intake was established by adding corn, olive or soybean oil, 21 26 while others implemented canola oil-based margarine.
Negative controls were established by adding the same volume of control-lentivirus (i.e., containing no exogenous gene) for 48 h, and incubating the fibroblasts with (group EmptyLPS) or without (group Empty) LPS for 72 h.
To assess the effect of PTEN overexpression and PI3-K-Akt pathway inhibition on LPS-induced lung fibroblast proliferation, the Pten-transfected group PTENLPS/Ly294002 was established by adding 50 μmol/L of the PI3-K inhibitor Ly294002 (CST, USA) to transfected cells for 1 h, followed by incubating with 1 μg/mL LPS for up to 72 h.
