Since the measurement time and cost increase proportionally as the increase of measurement points, it is essential to sample measurement points which can represent entire blade with sufficient confidence and accuracy.
In order to accurately monitor the gene expression changes, or transcriptional programming, that accompany these various stem growth transitions, it is essential to sample and pool stem tissues that are verifiably associated with specific stages of development.
Therefore, it is essential to sample relatively more herds and fewer animals within a herd than in a situation without clustering of disease events, as was shown for estimating the population prevalence for pseudorabies virus infection (14 ).
Also, as previously discussed, it may be essential to profile samples harvested at multiple time points during the course of therapy in order to fully capture the variability and extent of dynamic responses specific to a given therapy.
This result will be essential to guiding subsequent leaf sample collection and also indicates that very careful consideration should be given to sample collection not only for morphological analysis but also for other purposes such as physiology, transcriptomics and metabolomics, as leaf development appears to be highly plastic in aspen.
Specifically, it will be essential to evaluate markers in serum samples obtained prior to disease detection, in samples from women with clinically occult, localized serous cancers.
A standardized approach for sampling, storing, and processing DBS samples would be essential to allow successful implementation.
Future studies which develop effective tools to identify and define the types of bullying behavior present in a given sample will be essential to allow for the implementation of prevention programs targeting bullying in school environments.
Future studies focusing on the development of effective tools to identify and define the types of bullying behavior present in different samples will be essential to guide the implementation of prevention programs targeting bullying in school environments.
Accurate normalization is essential to eliminate sample-to-sample variations and to obtain reliable results, particularly in relative gene expression studies [ 21] for which multiple normalization genes with three to five reference genes could be required [ 23].
