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Glucose metabolism has been shown to be essential for sperm capacitation and we speculated that pyruvate indirectly affected glycolytic ATP production.
We produced a KO mouse for Rnase10 (12) and report here that the encoded product is the first single epididymal secreted protein found to be essential for sperm maturation and normal male fertility, providing evidence that inactivation of function of a single epididymis-specific gene product may be a feasible strategy to develop a male contraceptive.
LDH-C has been shown to be essential for sperm motility (Rodriguez-Paez et al., 2002) and spermatozoa from mice carrying a targeted disruption of the LDH-C gene did not show tyrosine phosphorylation and hyperactive motility, resulting in impaired fertility (Odet et al., 2008).
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IZUMO1 is the only sperm protein which is proven to be essential for sperm-egg fusion.
As of today, IZUMO1 is the only sperm protein which is proven to be essential for sperm-egg fusion.
The polypeptide encoded by mouse Zp3 exon-7 containing sperm combing-site of ZP3 has been demonstrated to be essential for sperm-binding activity by in vitro functional experiments [ 8- 10].
Male reproductive fluids contain a variety of biochemical components that are essential for sperm functions.
Under normal conditions, sperm capacitation take place in the female genital tract, however, the process can be mimicked in vitro by incubating epididymal or ejaculated sperm in a defined medium [18] [21], and a protein source that usually is bovine serum albumin (BSA) which approximates that of oviduct fluid is essential for sperm capacitation [43].
Glycolysis is essential for sperm motility and fertilization.
Sperm motility is essential for sperm transport in female reproductive tract.
Importantly, we demonstrated that proper turnover of certain key piRNA targets is essential for sperm formation.
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