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The correct conformation of Clp1 induced by ATP binding may be essential for interactions with other protein factors in the machinery, including Ssu72, Ysh1, Pta1, and Rna14.
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While DevRC interaction with DNA oligonucleotides containing two consensus binding sequences was shown by crystal structure analysis [18], phosphorylation of intact DevR at Asp54 was found to be essential for interaction with DNA [20].
While the conserved N-terminal region is recognised to be essential for interaction with ferroportin [ 18], the role of the C-terminal side is not well defined.
Any of these apparently small structural differences may be essential for interaction of Dil with its intracellularly or extracellularly accessible binding sites.
However, FOXP3 exon 2, which encodes for a portion of the FOXP3 repressor domain, has been specifically shown to be essential for interaction with and repression of retinoic acid related orphan receptors α and γt (RORα, RORγt) [ 34, 35].
Our recent resolution of the crystal structure of the BtpA TIR domain reveals that the motif positions a glycine residue that has previously been shown to be essential for interaction of BtpA with microtubules.
Future studies should comprehensively examine the metal bioavailability in aquatic sediments, because this factor is essential for interactions between the abiotic and biotic components of an aquatic ecosystem [2].
ATP processing by MutL is essential for interactions with MutH and UvrD [4], [13].
Secreted proteins are essential for interactions with the environment, particularly for sessile organisms such as fungi.
We subsequently explored which functional groups in 1 are essential for interactions with CDC25B.
The G domain is highly conserved, typical of all GTPases [ 19], and the C domain is essential for interactions with eRF1 [ 11, 61, 62].
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