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Thus, the putative −10 element of the cryptic promoter sequence appeared to be essential for function, while the putative −35 element did not.
We have used random and site-directed mutagenesis to identify residues in VirS that are important for its function and have identified a region in the putative sensory domain of VirS that appeared to be essential for function.
Second, the degree to which the domain is open or closed appears to be essential for function.
Because AtpD has been shown to be essential for function of the ATP synthase complex in yeast [ 80] and bacteria [ 81, 82] and similarly, loss of atpD expression in Arabidopsis disabled photoautotrophic growth [ 83], we hypothesized that a functional replacement (or a highly diverged ortholog) must be present on the chloroplast or nuclear genome.
Similar(56)
In some cases these modifications are known to affect structure and are essential for function.
The Hsp90/Cdc37 chaperone system is essential for function of normal and neoplastic cells.
RNAs adopt diverse folded structures that are essential for function and thus play critical roles in cellular biology.
This result suggests that Osw3 has protease activity and that this activity is essential for function.
An apparently fixed relative arrangement of domains is essential for function in many proteins.
Post translational modifications (PTMs) occur in vast majority of proteins and are essential for function [15], [26].
Mutation of the conserved tryptophan residue in the MAVS CARD (W68A) that is essential for function, also impaired TRAF5 binding (Figure 3B).
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