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Using RNAi and temperature sensitive mutants, dynein activity was previously demonstrated to be essential for formation of the synaptonemal complex during C. elegans meiosis (Sato et al., 2009), which in turn is required for stable lengthwise association between homologous chromosomes.
In addition, the COX7a2-like protein (COX7a2l, also called supercomplex assembly factor I, SCAFI), originally identified as an estrogen-responsive element (Watanabe et al., 1998), was reported to be essential for formation of complex IV-containing supercomplexes (Lapuente-Brun et al., 2013) and stabilization of the respirasome (Ikeda et al., 2013).
In addition to its canonical function during replication initiation, metazoan Orc6 is also essential for cytokinesis (Prasanth et al., 2002; Chesnokov et al., 2003; Bernal and Venkitaraman, 2011), helps to control centrosome copy number and, along with other pre-RC components, appears to be essential for formation of primary cilia (Stiff et al., 2013).
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Knowledge of the overburden pressure at each point in the well (described by the overburden gradient, S) is essential for formation pressure calculations.
Nuclear envelope remnants are essential for formation of the male pronucleus.
FLO11 was essential for formation of the biofilm subpopulation.
Despite this, these same studies found that centrioles are essential for formation of astral microtubules and cilia.
This shows that Fyn is essential for formation of the normal number of myelin sheaths per oligodendrocyte.
Moreover, proper regulation of the reversal frequency is essential for formation of both cellular patterns (Blackhart and Zusman, 1985).
Both ROS and Nox proteins are essential for formation and function of invadopodia, inducing ECM degradation and cell invasiveness.
The glycosylation of envelope proteins affects their folding and thus is essential for formation and immunogenicity of VLPs [ 10, 11].
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CEO of Professional Science Editing for Scientists @ prosciediting.com