This is potentially consistent with KIF17 motor activity promoting cililary D1R concentration, but not being essential for ciliary D1R access.
We were unable to detect a requirement for TULP3 or BBSome components, although these are essential for ciliary localization of SSTR3 and MCHR1 (Berbari et al., 2008b; Jin et al., 2010; Mukhopadhyay et al., 2010).
In vitro analysis further suggests that C2CD3 is required for recruiting the intraflagellar transport proteins IFT88 and IFT52 to the mother centriole, and is essential for ciliary vesicle docking to the mother centriole (Ye et al., 2014).
Interestingly, neither the entire N-terminus nor the ∼600 amino acid region at the C-terminus of Gli2 is essential for the ciliary localization; however, loss of both prevents the ciliary localization of Gli2.
Interestingly, we observed no ciliary accumulation of Sufu in Gli mutant cells, suggesting that Gli proteins are essential for the ciliary localization of endogenous Sufu (Fig. 5E and Table 3; n = 0/30).
A central region immediately C-terminal to the zinc-finger domains is essential for the ciliary localization of the Gli2 protein.
We next examined whether the region lying immediately C-terminal to the zinc-finger domains is essential for the ciliary localization of Gli2.
Ciliary compartmentalisation is essential for function, and the transition zone (TZ), found at the proximal end of the cilium, has recently emerged as a key player in regulating this process.
This article highlights the role of the Arf and Rab guanosine-triphosphatases and their regulators, as well as soluble N-ethylmaleimide-sensitive factor attachment protein receptor proteins, in polarized trafficking and ciliary targeting of rhodopsin, which is essential for the establishment and maintenance of photoreceptor polarity and health.
