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The problems can be enumerated with a prim scowl and a wagging finger.
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The bacterial suspension in PBS was enumerated with a UV-visible spectrophotometer and inactivated with 1% (v/v) formalin for 1 h at 30°C.
Bead numbers were enumerated with a Reichert Bright-Line Metallized Hemacytometer from Hausser Scientific (Horsham, PA).
Samples were enumerated with a hemocytometer for total leukocyte number.
Colonies were enumerated with a laser colony counter (Spiral System) after 48 h of incubation at 37°C.
Third, the S1-S2 components were enumerated with a 3′-dT20 appendage.
The number of positive cells was enumerated, with a cell being scored positive if 50% or more of their nucleus stained positive for NF-κB p65.
Total leukocytes in BALF were enumerated with a hemocytometer, and fractions of eosinophils, neutrophils, macrophages, and lymphocytes were determined in a cytospin sample stained with Diff-Quick.
Conidia from a MAT1-1 isoland and a MAT1-2 isolate were enumerated with a hemocytometer and used to inoculate 50-ml liquid cultures of Champe's medium at a concentration of 1 × 10 conidia per ml.
PBMC were enumerated with an automated Guava cell counter (Millipore, Billerica, MA).
The agar plates were incubated for 28 days at 37°C/5% CO2 and individual M. avium colonies were enumerated with an inverted microscope after 14 and 28 days of incubation.
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