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Time-kill assay indicated that S. maltophilia ATCC 13637 cells grown in MHB maintained their viability for at least 24 h of incubation, whereas S. maltophilia treated with chlorogenic acid at increasing concentrations (8, 16, and 32 μg mL−1) killed more than 90% of the cells and no viable cells could be enumerated after 12 h of incubation.
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All strains were enumerated after 3, 5, 8 and 14 days at 15 °C.
Results: In the experimental HIV-1 infection model, 5579±4190 CD4+ T cells secreting HIV-1 antigen were enumerated after polyclonal activation.
Then, the LAB3 cells population decreased: 107 and 106 CFU mL−1 were enumerated after 12 days in alginate-caseinate matrix and in alginate matrix, respectively.
For CFU analysis on FL cells, 2 × 105 cells were isolated and plated in methylcellulose media containing growth factors (Stem Cell Technologies), and colonies were enumerated after 12 days of growth.
For CFU assays, 2 × 105 GFP+ lentivirus-transduced HSPCs were isolated and plated in MethoCult GF M3434 media containing growth factors (Stem Cell Technologies), and colonies were enumerated after 12 days.
When plating mixed cultures, selective BHI agar plates with Listeria mono Selective Supplement I (Sigma-aldrich, Diegem, Belgium) were used for selectively culturing Streptococci at 37°C, whilst K. oxytoca was enumerated after incubating standard BHI plates at room temperature, as Streptococci were not able to grow at room temperature.
Colonies were enumerated after 21-days incubation at 37°C.
Y. pestis colonies were enumerated after 48 h of incubation at 28°C.
Bacteria were enumerated after plating a dilution series onto LB agar.
Plaques were enumerated after incubation at 35°C and 5% CO2 for 2 days.
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