TRAbs without stimulating activity can be enriched from normal individuals [11].
By means of a non-obese diabetic/severe combined immunodeficiency disease (NOD/SCID) xenotransplant assay in combination with specific cell surface markers (CD44+CD24-/low CD44+CD24-/lowriCSCs from metastatic and primary breast tumors and were shown to havenrichedility to reestablish tumor heterogeneity afrom transplantation [ 1].
6.5 weeks post-injection, the primary tumors were harvested, ECM proteins were enriched from tumors using the subcellular fractionation protocol described previously (Naba et al. 2012), and the composition of the ECM-enriched fractions obtained was characterized by mass spectrometry.
The c-kitpos CSCs were enriched from the CD45neg fraction, through incubation with a rabbit anti-c-kit primary antibody (Santa Cruz), followed by goat anti-rabbit antibody conjugated with magnetic microbeads for separation and isolation by AUTOMACS Technology (Miltenyi).
Malignant breast epithelial cells of 50 freshly isolated primary infiltrating ductal carcinomas of histological grade 2 and 3 were enriched from disaggregated tumour tissue as described previously [ 9].
American dance is enriched from coast to coast.
Photosynthetic bacterial inoculum was enriched from a water sample collected from a pond.
LMs were enriched from lung cell suspensions by adherence.
Part of the RNA samples from each condition was enriched for primary transcripts by incubation with Terminator exonuclease (TEX), which only degrades processed RNA molecules that carry a 5′-monophosphate but not primary transcripts that have a 5′-triphosphate.
In addition, differential RNA-seq was applied, that is, part of the RNA samples from each condition was enriched for primary transcripts, allowing genome-wide identification of transcription start sites (TSSs), and hence, of promoter regions and 5′-untranslated regions (5′-UTRs) of mRNAs (Sharma et al. 2010).
