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Analyses of several GEM mammary cancer models suggested that tumors from certain models may be enriched for cells with stem cell characteristics compared with other models.
These different size tumor cell subpopulations may represent cells enriched at different phases of the cell cycle, smaller cells tend to be enriched for cells in G0 and larger cells tend to be enriched for cells in G2/M (not shown).
Accordingly, cells with p75NTR high expression are considered to be enriched for cells with a less differentiated phenotype (stem cell properties), whereas the cells with low P75NTR expression are enriched for cells with a more differentiated phenotype.
According to this method, cells adhering most rapidly to collagen IV are considered to be enriched for cells with a less differentiated phenotype (stem cell properties); whereas the late adherent cell population contains relatively fewer cells with stem cell properties and the non-adherent cell population consists of cells with a more differentiated phenotype.
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We hypothesized that the interaction partners of the baits would be enriched for cell cycle regulators relative to random proteins.
To identify a subset of the negative genes that were likely to be enriched for cell cycle regulators, we performed a virtual protein-protein interaction screen to find proteins that interact with known or suspected cell cycle regulators.
Consistent with the previous analysis run on dog samples only, genes with higher expression in tumors (positive correlation with first PC) were found to be enriched for cell cycle (P = 6e-9) anDNANA replication (P = 7e-6) related genes.
CD27+ cells were enriched for cells expressing IgG, IgM, Bcl2 and CD95 but not CD44.
In contrast, clusters 1 and 2 are enriched for cells with EGFR mutations; within each of these clusters, cells with wt and mutant EGFR are segregated.
Peripheral blood samples were enriched for cells expressing the epithelial-cell adhesion molecule (EPCAM) with antibody-coated magnetic beads, and cells were labeled with the fluorescent nucleic acid dye 4,2-diamidino-2-phenylindole dihydrochloride.
In the present work, we tested if anchorage-independent cell culture techniques allow the generation of spheroid-cultures and if these cultures were enriched for cells with functional and phenotypic properties characterizing CSC of HNSCC.
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be made for cells
be provided for cells
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be spared for cells
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be needed for cells
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