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Breast T-ICs (BT-ICs) can be enriched by purifying CD44+CD24-/low CD44+CD24-/lowng or by purifying sphericellslusters of self-replicating cells (mammospheres) from cell suspensions.
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I would be enriched by volunteering.
A second adapter was then ligated to the MmeI cut site, and the adapter-ligated cDNA construct was enriched by PCR and purified (Additional file 1: Figure S1).
The glycated peptides were enriched by boronate affinity chromatography, purified by RP HPLC, and the fractions were examined for radioactivity.
The required fragments were enriched by PCR amplification and purified using a Qiagen MiniElute PCR Purification Kit.
After obtaining a 92 bp DNA band on 6% denaturing PAGE gels, the PCR products were enriched by ethanol precipitation and purified using Spin-X filter columns (Fisher).
Cell populations for adoptive transfer were not purified, but were enriched by the culture procedure.
The ligation products were enriched by PCR amplification (15 cycles) and purified by 6% TBE PAGE Gel electrophoresis.
The final library was enriched by a 10-cycle PCR and purified by AMPure bead clean-up.
Following 3' adapter ligation, the resulting library was enriched by PCR amplification (15 cycles), and purified by PAGE.
Crude mitochondria were enriched by differential centrifugation, and were further purified by centrifugation in a 20-34% Nycodenz density gradient at 52,000 g for 90 min.
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