This new rice has been engineered, using a daffodil gene, to produce beta-carotene, a nutrient the body can convert into vitamin A. Watching the pitch, you can almost feel the moral ground shifting under your feet.
Fibrin micro-beads were engineered using a droplet microfluidic system.
A laccase, CotA, from Bacillus subtilis was engineered using a combination of rational and directed evolution approaches.
Nitrogen - rich graphitic carbon nitride (Ng-C3N4) with improved photocatalytic activity was engineered using a facile post-annealing treatment of pristine g-C3N4 in N2 atmosphere.
Generally speaking, ligand-specific protein fragments have been engineered using a two-fold strategy: i) creation of a library of protein variants via targeted or random mutagenesis of the parent protein, and ii) selection of target ligand binders via a phenotypic selection such as phage display, yeast surface display or ribosome/mRNA display.
In order to obtain improved enzymes and understand the structure-function relationship better, Man26P was engineered using a modified DNA shuffling method and a 96-well plate high-throughput screening technology.
The bioreporter was engineered using a series of pBEVY yeast expression vectors that allowed for bi-directional constitutive or inducible expression of the individual luxA, B, C, and E genes.
Recombinant fragments of the LevT, LevQ and LevS proteins were engineered using a vector pQE30 (Qiagen, Valencia, CA) by in-frame fusion of an N-terminal 6×His-tag to the putative sugar-binding domains of LevT (beginning with Thr41) and LevQ (starting with Gly40), or to the C-terminal histidine kinase domain of LevS (at Asn214), respectively.
EGFP-expressing P. agglomerans containing microparticles were engineered using a 1, 2 or 3%% alginate as described above.
