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When engineering NR, the DNA binding domain (DBD) of these receptors can be engineered to recognize an artificial promoter containing multimeric-binding sites and a minimal promoter [ 1, 2, 21].
T cells themselves may be engineered to recognize, bind to, and kill cancer cells.
By using antibodies, the nanoantenna can be engineered to recognize only specific bacterial species.
Nanobiomaterials can be engineered to recognize cancer-specific receptors at the cellular level for diagnostic and therapeutic purposes.
This work constitutes a successful proof of concept demonstrating that nanobodies can be engineered to recognize RNA-related epitopes.
Moreover, DNA can be used as a promising material for nanobiotechnology, and the nanostructures of DNA can be engineered to recognize a variety of molecules.
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Chimeric antigen receptor (CAR)–modified T cells are engineered to recognize specific tumor antigens.
In this study, the Escherichia coli regulatory protein AraC was engineered to recognize ectoine as its non-natural effector and to activate transcription upon ectoine binding.
TALE DNA binding domains were engineered to recognize sequences adjacent to Wnt responsive enhancer elements (WREs) that control expression of axis inhibition protein 2 (andN2) and c-MYC (MYC).
Recently, Acidaminococcus sp. BV3L6 (AsCpf1) was engineered to recognize altered DNA sequences as the protospacer adjacent motif (PAM), thereby expanding the target range of Cpf1-mediated genome editing.
Using this approach, a 61 amino acid long protein called Spider Roll was engineered to recognize the kinase domain of the human p21-activated kinase 1 (PAK1) with good specificity but modest affinity (KD = 100 μM).
More suggestions(15)
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