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Microbial oils are gaining importance because they can be engineered to accumulate lipids enriched in desired fatty acids.
Alternatively, plants might be engineered to accumulate additional storage compounds than naturally occurring.
There are several technical or scientific challenges that must be met before oilseed crops can be engineered to accumulate adequate levels of LC-PUFA.
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C. glutamicum was engineered to accumulate L-citrulline as major product, both from glucose as well as from the alternative carbon sources starch, glucosamine and xylose.
Here we report that construction of a V. cholerae O1 classical strain, O395-N1-E1, that has been engineered to accumulate CTB in the periplasmic fraction by disrupting the epsE gene of type II secretion pathway.
In reporter assays using the luciferase gene under control of the human c-fos 5′-upstream region from −2.2 kb to +1, expression of Hic-5, that was engineered to accumulate in the nucleus, stimulated the transcriptional activity of the c-fos enhancer.
Recently, Camelina seed oil was engineered to accumulate EPA and DHA [ 19 ] — in this study, the authors report the highest levels of C20+ omega-3 LC-PUFAs in a recognised oilseed crop — 31% EPA or 25% EPA plus DHA.
Microbes can be engineered to consume various carbon sources, and accumulate increased amounts of fatty acids with different composition.
First, NSCs can be engineered to produce different genes.
Recently, plants have been engineered to detoxify and accumulate As in the aboveground tissues.
When these mice were engineered to lose their ability to accumulate MDSC in tumor-bearing hosts (S100A9 knockout), growth of the immunogenic MM cells was significantly reduced showing again that the accumulation of MDSC at early stages of MM plays a critical role in MM progression [ 87].
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