Two genetic systems were engineered in which mammalian cells either overexpressed DNase II or were knocked out for the enzyme.
A mutant human class IV enzyme was engineered in which a Gly residue was inserted at position 117 (G117ins).
Next, a tricomponent complex was engineered in which the three molecular entities (the D3 antigen, COMP coiled-coil domain, and Z domain) were genetically connected in tandem to create the D3 COMP Z tricomponent complex, or its reversal oriented construct, Z COMP D3.
To understand the influence of these aromatic amino acids and the associated high hydrophobicity, apoA-I variants were engineered in which aliphatic amino acids were substituted with or without causing a decrease in overall hydrophobicity.
A Modoc/yellow fever chimeric virus was engineered in which the structural prM and E genes of yellow fever virus 17D were replaced by the homologous genes of Modoc virus.
To this end, a construct encoding the invariant chain (Ii) was engineered in which the Class II associated invariant chain peptide (CLIP) sequence was replaced by an immunogenic epitope derived form Heat Shock Protein 60, HSP60 178–186.
A series of mutant carriers were engineered in which the interaction energy of the cytoplasmic network increased or decreased compared to the wild-type.
Additionally, using the DOCK-AND LOCK™ (DOCK-AND LOCK™ technology [ 17, 18], a hexavalent form of hR1 (Hex-hR1) was engineereDNL™ which four hR1 Fabs were linked to hR1 IgG [ 16].
A chimeric cytokine receptor named 4αβ was engineered in which the IL-4 receptor α (IL-4Rα) ectodomain was fused to the shared βc subunit, used by IL-2/IL-15.
Once the Focus, new Taurus and 500 are all out, only such relatively low-volume Ford models as the Mustang, Crown Victoria and Lincoln will continue to be engineered in the U.S. At GM, which is more reliant on cars and has many more models and brands, the export of engineering is only somewhat less extreme.
