The baculoviruses are engineered by inserting a mammalian expression cassette for delivering foreign genes in mammalian cells.
This receptor was engineered by inserting a C-terminal rho1D4 epitope tag and an N-terminal FLAG epitope tag to allow its purification and detection.
Here we report a novel multifunctional green fluorescent protein (mfGFP) tag which was engineered by inserting multiple peptide tags, i.e., octa-histidine (8×His), streptavidin-binding peptide (SBP), and c-Myc tag, in tandem into a loop of GFP.
The mfGFP was engineered by inserting three tags (a total of 67 residues including linkers) into GFP in tandem: octa-histidine (8×His) and streptavidin-binding peptide (SBP) for affinity isolation, and c-Myc for immunological detection (see Supplementary Figure S1).
The model was engineered by inserting the LacZ reporter gene, which encodes β-galactosidase into the Slc9a6 genomic locus (Deltagen).
Expression plasmids for KirCII, ACP4, and ACP5 (pFW139 pFW139, and pFW140 respectively) were engineered by inserting the corresponding NdeI– EcoRI fragments into a pET28 vector.
The pEGFP-SHP2 WT and C/S mutant were engineered by inserting a coding region into the SalI and BamHI sites of pEGFP vector (Stratagene).
A vector expressing dominant negative Akt1 was engineered by inserting a K179M mutant dominant negative Akt1 cDNA (Millipore) into a multiple cloning site of a eukaryotic expression vector, pUSEamp (Millipore).
In summary, the NSe strain c-DNA infectious clone (derived from the MV-Edm vaccine lineage Seed B) [ 36] was engineered by inserting the human CEA gene upstream of the MV N gene.
