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After the collection of tumors and organs, each of these was divided into two pieces so that both methods could be employed for quantification of the probe in each organ.
The results have shown that, over the concentration range 1×10−3 1×10−5 M, the reduction stripping pattern, provided by a simple CV at low scan rate, can be employed for quantification of these analytes, while for more dilute solutions, LSASV is necessary.
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The capillary voltage was set to 4000 V. MRM was employed for quantification.
A multivariate calibration strategy based on inverse least squares approach was employed for quantification.
Multiple-reaction monitoring was employed for quantification while switching the electrospray ion source polarity between positive and negative modes.
Multiple-reaction monitoring (MRM) scanning was employed for quantification with switching electrospray ion source polarity between positive and negative modes in a single run of 16 min.
The d0/d3-MPIA-aldehydes were monitored by the transitions of [M+H]+→m/z 322 and [M+H]+→m/z 165, and the obtained detection limits were in the range of 0.18 15.9 pg/mL at signal to noise ratio of 3. The global isotope internal standard technology was employed for quantification analysis with d3-MPIA-aldehyde as internal standard for corresponding d0-MPIA-aldehyde.
A relative quantification method was employed for quantification of target molecules by calculating the ratio between the amount of the target molecule and a reference molecule within the same sample, according to the manufacturer's protocol.
The comparative Ct method was employed for quantification of transcripts according to the manufacturer's protocol.
The target ion was employed for quantification, and the confirmation ion from the same isotopic cluster was used to confirm the identity of the compound.
The terminal deoxynucleotidyl transferase (TUNEL) reactivity assay (ApopTag, S7100, Intergen, NY, USA) was employed for quantification of apoptosis (Gavrieli et al, 1992).
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