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In another example of how this 3-component system can be employed for functional cloning, we expressed low levels pro-Caspase-10 and high levels of Fas, then screened cDNA libraries to clone adaptor molecules that link Fas to Caspase-10 (Figures S12 S15).
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Then, Gibbs sampling is employed for functional inference of the proteins with unknown function ("unannotated proteins").
vesca 'Hawaii 4' was employed for functional analysis of expressed DNA sequences initially identified as being unique to Fragaria and of unknown or poorly understood function.
Integrative analysis of multiple omic datasets (e.g., interaction networks and gene expression) has the potential to elucidate the intricate interactions involved in biological processes, and has been employed for functional annotation [ 1], active module discovery [ 2], and biomarker discovery [ 3].
We also recall the following known result by Ben-Naoum, Troestler and Willem, which will be employed for the energy functional.
Moreover, we describe a useful pipeline of LRRK2-related assays that can be employed for the rigorous functional validation of LRRK2-interacting proteins.
The oxidized functional group may be the end point of labeling, but oxidation methods can also be employed for the creation of functional groups for secondary labeling, e.g., biotinylation for affinity purification or fluorescent labeling for detection.
Superinfection assays should be employed for very sensitive detection of functional receptors on the cell surface.
DAVID functional annotation clustering was employed for the functional annotation of the mRNAs.
Blast2GO [ 81] was employed for GO functional categories according to the Gene Ontology terms molecular function, cellular component and biological process.
Clonal cell lines were evaluated for PS1 and PS2 protein expression by Western blot analysis (Fig. 2A) and those lines expressing at least a 50% decline were employed for subsequent functional studies.
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