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In this study we have shown that kita expressing melanoblasts can be efficiently transformed by the HRAS oncogene in the presence of active p53 and give rise to melanoma with a higher efficiency and much lower latency than mitfa expressing melanoblasts and melanocytes.
Immortal fibroblasts also required DDX21 expression to be efficiently transformed by oncogenic RasV12 alleles.
To directly test whether FBXO28 activity is required for MYC-induced transformation, we engineered P53−/− immortalized mouse embryonic fibroblasts (MEFs) (which are known to be efficiently transformed by MYC (Ecker et al, 2009), with retroviruses encoding MYC and ΔF-FBXO28, or the individual genes, and performed transformation assays.
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Primary human amniocytes are efficiently transformed by adenoviral E1-functions.
Cyclic substrates were also evaluated, and 1-cyano-1,2,3,4-tetrahydroisoquinolines 4 a– b were efficiently transformed by using different lipase preparations.
The resulting Δ cpaAIR gene disruption mutant should not produce a functional CpaAI Type II restriction endonuclease and, therefore, should be efficiently transformed with plasmid DNA lacking methylation by the FnuDIIM methyltransferase (5′-m5CGCG-3′) [ 15].
It is not known whether the product P might be efficiently transformed into other end-products or the product E synthesized by another P450 gene (F3′H).
Primary amniocytes from routine amniocentesis samples can be efficiently transformed with adenoviral functions resulting in stable human cell lines.
The overall CP-OFDM system can be efficiently realized by fast Fourier transform (FFT/IFFTs), and one important advantage is that the CP-OFDM can maintain a full orthogonality, which requires only a simple equalizer at the receiver.
Accordingly, by solely converting the HA cleavage site motif, FPV can be efficiently attenuated and transformed into a non-pathogenic avian virus.
The above property means that the watermarking LUT can be efficiently computed by relying on a fast Fourier transform.
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