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Although some genes are regulated by Pol II pausing in close proximity to the TSS [ 65], others are reported to be efficiently transcribed into precursor transcripts, while the efficiency of transcript processing is being regulated [ 66, 67].
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The relatively small genomic DNA (3.4 × 10 bp) is efficiently transcribed (90%) into about 12,500 genes, some of which show analogies with human genes.
The wheat P450 cDNAs were efficiently transcribed, but no protein or activity was found.
As shown in Figures 1A and 1C, all templates were efficiently transcribed (compare lanes 1 and 2).
The PTH gene was efficiently transcribed, processed and translated to mature immunoreactive PTH.
This "replicational selection" should guarantee that genes on the leading strand, particularly the essential ones, are efficiently transcribed [ 5- 8].
These findings support the idea that uAUG genes are less efficiently transcribed than uAUG-less genes.
HMGCLL1 is known to be transcribed into various alternative isoforms.
Rarer targets can be more efficiently reverse transcribed into cDNA this way; Promega technical notes suggest the use of 3.125 U/ l for reverse transcription instead of 1.25 U/ l in this regard.
This suggests that the exon 1 3 mRNA is either not efficiently transcribed or unstable.
Importantly, H2be is efficiently incorporated into nucleosomes, preferentially within transcribed genes, thereby mediating a rather global change in chromatin composition.
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