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In conclusion, in a rat model for liver fibrosis M6P-HSA-liposomes can be efficiently targeted to non-parenchymal cells, including HSC.
One might argue that in order to have positive effects, a program must be very clear about what teachers are expected to do, so that professional development and coaching can be efficiently targeted to helping teachers do those things.
To be efficiently targeted to the proteasome, substrate proteins have to be attached to a poly-ubiquitin chain, in which at least four ubiquitin molecules are successively linked through specific lysine residues of ubiquitin (e.g., K48 and K11) [7], [7].
Adrian et al. [ 134] confirmed that M6P-HSA-liposomes can be efficiently targeted to non-parenchymal cells, including HSC.
Fusions of this protein with respective N-terminal signal sequences can be efficiently targeted to mitochondria and peroxisomes.
We and others have established that the Green Fluorescent Protein (GFP) can be efficiently targeted to the nucleus by fusion to topogenic sequences [ 19- 23].
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Thus, we conclude that, despite the addition of a GFP tag, GFP-L22 is efficiently targeted to nucleoli and incorporated into cytoplasmic ribosomes.
As can be seen in Figure 3C, the mito-Tks5-GFP construct was efficiently targeted to mitochondria and localised extensively with mito-tracker red staining in the myoblast cells.
Our preliminary results indicate that the Somatostatin type 3 receptor (SST3R), a GPCR described in neuronal PC [8], was efficiently targeted to the axoneme in βarr-deficient cells (Figure S11).
At E16, immunogold particles labeling the receptor were visualized by electron microscopy at the inner side of plasma membrane of round or elongated-shaped cells in the EGL (Fig. 3D,E), suggesting that receptors are efficiently targeted to active sites and could be functional.
(F ) The GFP-eS26D33N fusion protein is efficiently targeted to the nucleus.
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