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If two adjacent exons get too close to each other, the intron between them cannot be efficiently spliced out by the spliceosome.
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Subsequently, we confirmed the efficacy of two cited3 MOs by RT-PCR: injection of each cited3 MO prevented the splicing of the first intron significantly, whereas it is efficiently spliced out in the embryos injected with the control MO (supplementary material Fig. S2).
These introns appear to be efficiently spliced in embryonic brain, as might be expected after the downregulation of PTBP1.
ILEs were shown to be efficiently spliced but to share specific features compared with resident introns, such as a bigger size and a conserved secondary structure.
This correlation is not particularly strong and have various explanations, for example, longer introns may be more efficiently spliced out or may be splicing is important for an efficient transport of mRNA.
Remarkably, Xist transcripts were efficiently spliced after nuclear transfer (Supplementary Figure S4C).
The high expression level of the Act1 promoter in Physcomitrella indicates that this intron is efficiently spliced by moss cells.
Furthermore, the transcript of the putative substrate of GmIREs, GmbZIP68, harbors a canonical site for IRE1 endonuclease activity and was efficiently spliced under ER stress conditions.
Through alterative splicing, exon 16 can be spliced out, giving rise to the β -variant of ADAM33.
The introns are spliced out and subject to degradation during mRNA maturation.
Moreover, Mtz can be efficiently washed out, the affected tissue can quickly recover.
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