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Indeed, in a strain carrying a temperature-sensitive rsp5 allele, Def1 failed to be efficiently processed in response to 4-NQO at the restrictive temperature, whereas Def1 processing and accompanying Rpb1 degradation was observed in the WT control strain.
In our previous studies [8, 9], we have shown that these large chemical libraries can be efficiently processed in parallel using Apache Spark [10] and scales well with increasing computation power.
To this end, we have participated in development of tools that allow bioinformatics data to be efficiently processed in Hadoop: Hadoop-BAM and SeqPig [ 30, 31].
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In this parallel algorithm, the system of linear tridiagonal equations, vector-vector additions and constant-vector multiplications are efficiently processed in parallel.
Furthermore, due to its metabolic activation, 35b was efficiently processed in liver cells compared to other cell types, including human cardiomyocytes.
Our data demonstrate that the amiRNA precursor is efficiently processed in planta in all tissues tested and the amiRNA-guided cleavage of target transcripts is highly specific.
Therefore, it is reasonable to propose that the N-terminal methionine of the rArtinM was efficiently processed in E. coli BL21-CodonPlus DE3 -RP BL21-CodonPlus DE3 -RP
Altogether, these results demonstrate the existence of a calpain site nearby Kidins220 C-terminus that is efficiently processed in vivo after protease activation and produces a 15-kDa fragment of low stability.
Analysis of the primary miRNA transcript level using RT-qPCR showed an enrichment of these transcripts for the chimeric constructs, suggesting that the murine flanking regions used in this study result in structural changes that cannot be efficiently processed by Drosha/DGCR8 in the nuclear processing step.
However, complex object relationships, as, for example, lineage trees of dividing cell populations that can comprise millions of cell objects, cannot be efficiently processed when stored in textual data formats such as Extensible Markup Language used in OME-TIFF and semantically typed data hypercubes.
In addition, we identified precursor transcripts for the 6 miRNAs expressed in rice, showing that these precursors can be efficiently processed using a transient expression assay in transfected Nicotiana benthamiana leaves.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com