Here we show that self-renewal of Sox1-positive primitive NS cells derived from mouse, rat and human can be efficiently maintained in serum-free N2B27 medium supplemented with LIF/CHIR/Y.
Additionally, based on the data shown above, we speculated that even if the host's repair capacity is improved, hESCs themselves will not be efficiently maintained or expanded in the context of old systemic and local organ environments, and will not directly contribute to the repair of aged skeletal muscle.
"There are some areas of code in running programs," he writes, "that may as well be marked Here Be Dragons, and there are some programs that have run for decades — at universities, corporations, banks — that cannot be efficiently maintained or enhanced because nobody completely understands how they work".
This showed that the aggregate was efficiently maintained as one compartment, and whenever new foci emerged, they typically merged soon after with the pre-existing deposit.
This deposit displays limited exchange with the cytoplasm (assessed by Hsp104), is efficiently maintained as a single compartment, and is faithfully inherited by the aging mother cell.
We observed that vaccination heterologously resulted in an enhanced DTH and splenocyte proliferation after infection, and the responses were efficiently maintained at long-term.
Dioguardi (2008) also reported that collagen synthesis is efficiently maintained only when specific amino acids are continuously available and present in a specific ratio.
The cells were efficiently maintained in these culture conditions, presumably because of support from autocrine chemo-cytokines and growth factors (see below), and developed larger-sized adherent cells (Fig 2D).
Since AchR clustering is efficiently maintained in MCK-UCP1 mice, we assessed whether axonal regeneration upon injury was normal.
These differences also support the hypothesis that CIV is a separate virus lineage, which is efficiently maintained in the dog population by horizontal dog-to-dog transmission.
