To our surprise, both variants mimicking phosphorylated and non-phosphorylated forms of Gli2 are efficiently localized to the cilia and their ciliary localization can both be inhibited by PKA.
Further, when the longin domain of VAMP7 was attached via a linker (GSGGSG) to the neutral reporter CD8, the resulting construct was efficiently localized to lgp110-positive late endosomes/lysosomes.
In contrast, the T3N-QscR HTH-Myc protein was efficiently localized to the nucleus as indicated by the overlap between staining in the immunofluorescence and DAPI images (Fig. 2).
By applying a robust approach based upon an iterative, weighted, cost-minimization algorithm known as stress-majorization [18, 19], it is shown that a cluster of sensor nodes can be efficiently localized in the absence of complete pairwise distance measurements.
Human TDP1 contains two putative NLSs at residues 56 74 of the non-essential N-terminal region and residues 216 223 within its TDP domain, and must be efficiently localized and expressed in the nucleus for repair mechanisms [ 36, 37].
The cooperative action of the tripartite NLS ensures that human MSH6 is efficiently localized into the nucleus.
Similarly, another tetraspan protein claudin-14, was less efficiently localized to specific membrane domains when palmitoylation was abolished by site-directed mutagenesis (Van Itallie, 2005).
Transient exposure of human sarcoma cell line, containing Cre-dependent lacZ reporter, resulted in efficient recombination in the host chromosome, indicating that the bacterially delivered protein was not only efficiently localized to the nucleus but also retained its biological function.
The Imp3Q protein appeared less efficiently localized to the nucleus than the Imp3 protein (Figure 3).
In contrast, the STAT1-CC-GFP construct efficiently localized to the nucleus within 30 minutes of IFN-γ addition in both sensitive and resistant cell lines.
