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Our data demonstrated that liver lymphocytes can be efficiently isolated by simple perfusion.
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In all cases one diastereoisomer is detected in the crude reaction mixture and the products are efficiently isolated by direct recrystallization.
In the current study, LDSCs were efficiently isolated by a shortened protocol that limited the usage of enzyme cocktails like collagenase and hyaluronidase and also with minimal exposure to enzyme digestion time.
Thus, anti-tumor circulating PBMC-derived T cells could be efficiently isolated from melanoma patients by our novel ex vivo enrichment protocol.
HRTC were efficiently isolated from clear cell and chromophobe RCC and characterized by their flattened polygonal morphology and presence of lipid vesicles in the cytoplasm.
Although wild-type Fas GFP was efficiently isolated, we did not detect GRASP65 in these immune complexes.
Expression and secretion of RANTES can be efficiently induced in isolated HSCs by stimulation with TNFα or IL-1β via NF-kappaB and JNK signaling pathways (Schwabe et al. 2003).
Alternatively, subtractive hybridization may isolate those transcripts that can not be efficiently detected by SAGE, for instance transcripts lacking an NlaIII restriction site.
Human pathologic B cells isolated from patients with CLL (B-CLL) have been reported to be efficiently killed by 1 μM SAL, in contrast to peripheral blood mononuclear cells from healthy subjects.
This hypothesis is experimentally supported by the fact that resistance genes of serotype Typhimurium DT 104 can be efficiently transduced by P22-like phage ES18 and phage PDT17, which is released by all DT 104 isolates analyzed (14 ).
Such coherent expression modules should be efficiently extracted by EEM.
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