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First, we confirmed that with HVJ-liposome method fluorescence isothiocynate (FITC -labeled ODN could be eFITC -labeledtrODNcouldnto rat glomerular cells via renal artery.
For example, instead of developing a rational, flexible, and predictable regulatory process that would allow new nuclear technologies to be efficiently introduced into the market, the President introduced a program where government bureaucrats decide what type of nuclear technology would receive taxpayer money to offset development costs.
First, we show that a human NCAM isoform can be efficiently introduced into the forebrain and is correctly expressed and polysialylated.
Both the chemotherapeutant and as-miR-21 could be efficiently introduced into tumor cells.
Thus point mutations can be efficiently introduced into the genome by two sequential transformations with PCR fragments.
However, S. cerevisiae remains one of the few organisms in which point mutations can be efficiently introduced into the genome that are both "targeted" and "precise".
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The reporter genes were efficiently introduced into the CHO genome in a Cre-dependent manner, and transgene expression was detected after the integration reaction.
Moreover, using a cationic lipid reagent, the siRNAs were efficiently introduced into leukemia cell lines with t(8 21), SKNO-1 (30 40%) and Kasumi-1 (60 70%) cells, and reduced specifically the endogenous AML1 MTG8 expression.
The experimental results also demonstrated that antimiR-21 were efficiently introduced into the cells and knocked down miRNA-21 to inhibit its function, leading to growth inhibition and apoptotic cells death.
Random point mutations were efficiently introduced into small sized DNA encoding the signal peptide of glucoamylase and the resulting recombinant S. cerevisiae with beneficial point mutations in its signal peptide was able to secrete ca. 30% more glucoamylase than that with native signal peptide.
Real-time PCR results showed that anti-miR-31 was efficiently introduced into the cells and reduced miR-31 levels to 44.1% in HCT-116p53+/+ and 67.8% in HCT-116p53-/-cell line (p = 0.042 and 0.046).
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