ASCs changed to round-shaped adipocytes already around 2 days after induction, and at about 2 weeks, 95% of the ASCs were efficiently induced into adipocytes, whereas for UC-MSCs, clear morphology change began 5 days after induction and complete differentiation occurred three weeks after induction.
U937 monocytes can be differentiated into macrophages with phorbol myristate acetate (PMA) and COX-2 expression can be efficiently induced in U937 macrophages with a variety of stimuli, including LPS (schematically shown in Figure 4C) (Arias-Negrete et al., 1995).
We chose the concentrations at which S phase arrest can be efficiently induced (Figure 2).
RAW264.7 cells were efficiently induced to differentiate into osteoclasts within 72 h through stimulation with 10 ng/ml RANKL.
All IgG isotypes were efficiently induced by PA-Av-C3d3/IFA, indicating that sufficient T cell help was recruited to promote class switch recombination and continued differentiation into plasma cell effectors.
However transitions at CpGs in TP53 are efficiently induced by nitrosative DNA damage [31], [58], [59], [73], [74], [75].
As shown in Figure 4d, both cloned promoters were efficiently induced upon 3MBA exposure.
Donor cells that are difficult to reprogram can be more efficiently induced into the pluripotent state by adding hTERT and SV40 large T antigen to the reprogramming factors originally used by Yamanaka's laboratory (Park et al., 2008).
Like MTT1, MTT3 and MTT5 are more efficiently induced by Cd than Cu, and behave as multi-stress response proteins.
C5-Pro BiFC was most efficiently induced by IPAF.
