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Although addition of bFGF into this system may increase the number of NE cells, NE cells can be efficiently generated from hESC without bFGF.
Kyba et al. were the first to report that HSCs can be efficiently generated from ESCs in the OP9 co-culture system by combining this with an inducible HOXB4 expression system (OP9 and iHOXB4 system) [11].
Taken together, our results demonstrate that AP-positive colonies can be efficiently generated from NP and REF cells (approximately 0.2%) and approximately 20% of them became iPS-like colonies with the ESC morphology.
Moreover, hPGCL cells can be efficiently generated from hESCs maintained in medium containing the GSK3β inhibitor CHIR990021 (Irie et al., 2015).
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TRAP+ multinucleated cells (mean ± SEM cell number 210 ± 36 cells/well) were efficiently generated from SFMCs of gouty arthritis patients in the presence of 30 ng/ml of M-CSF and 10 ng/ml of RANKL.
Thus, despite intensive cytotoxic pretreatment, functional GD2-specific T cells were efficiently generated from an Ewing sarcoma patient and potently and specifically eliminated autologous tumour cells in vitro, confirming the clinical feasibility of GD2 targeting.
Taken together with these rat iPS cell derivation reports, our results demonstrate that iPS cells can be efficiently generated and maintained from genetically unmodified various rat tissues with different sets of factors.
Furthermore, the secret keystream can be efficiently generated by a keystream generator of stream ciphers.
This raises the following question: How can MPP+ be efficiently generated extracellularly in the absence of a system for its transport from the cell (Fig. 1C)?
Similar iNS cells were also efficiently generated from rat tail-tip fibroblasts using both retroviral and PiggyBac transposon systems.
ES-derived coat-color founder mice (100%) were also efficiently generated from B6/129 F1 hybrid ES (V6.5) cells under the serum- and feeder-free condition (8 100% ES-derived coat-color mice/42 embryos transferred, 23.8%; Table 1 and Fig. S5).
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