Both showed normal numbers of filopodia at their outer edges and could be efficiently expanded, dissociated and plated.
However, most tissue stem cells (e.g. hematopoietic and neural stem cells) cannot be efficiently expanded in culture by present technologies.
However, most adult stem cells, including hematopoietic stem cells and muscle stem cells, could not be efficiently expanded in vitro.
Nevertheless, HACDC can be efficiently expanded for up to 20 PD.
We have developed cell culture methods that allow primary porcine renal cells to be efficiently expanded while maintaining normal renal phenotype.
Similar to most of the adult stem cells, MuSCs can barely be efficiently expanded in vitro.
Human epidermal stem cells are privileged among adult (tissue) stem cells because they can be efficiently expanded ex vivo (Gallico et al, 1984; Rochat et al, 2013).
Even after allodepletion (using anti-CD25 immunotoxin), donor T cells could be efficiently transduced, expanded, and subsequently enriched by CD19 immunomagnetic selection to >90% purity.
Furthermore, we found that T cells could be efficiently stimulated and expanded by direct electroporation of PBMCs with mRNA encoding a chimeric membrane protein consisting of a single-chain variable fragment (scFv) against CD3 (OKT3) and the intracellular domains of CD28 and 4-1BB (OKthe28BB) in the presence of IL-2.
Additionally, based on the data shown above, we speculated that even if the host's repair capacity is improved, hESCs themselves will not be efficiently maintained or expanded in the context of old systemic and local organ environments, and will not directly contribute to the repair of aged skeletal muscle.
