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Maternal mRNAs in particular can be efficiently depleted using antisense deoxyoligonucleotides (oligos), mediated by endogenous RNase-H activity.
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We confirm that the oxygen was efficiently depleted using finite-element modelling to accurately recreate the electrochemical thin-layer cell.
As shown in Fig. 3A, LSD1 could be efficiently depleted in HeLa and SiHa cells.
In contrast to the unchanged level of ERK2 used as a loading control, PAIP2 is efficiently depleted by siRNA (a mean of 96% of inhibition), whatever the specific-siRNA sequence used.
Quantitative real time PCR showed that Mcph1 and Mcph1gt were efficiently depleted by >70% (Figure S3).
CD4 T cells are efficiently depleted by TLR7-ligands ([26] and Fig. 4A/B) and during IAV-infections [20].
Western blotting of whole cell extract after RNAi confirmed that each protein was efficiently depleted without affecting the levels of the other proteins (Fig. 5).
Non-crosslinkable DnaN-HT was efficiently depleted from agarose plugs during the entrapment assay.
While Rab3a was efficiently depleted from synaptic vesicles, Rab26 persisted on membranes.
BAG1 and BAG3 were efficiently depleted by short interfering RNAs (siRNAs).
Immunoblotting results clearly demonstrated that both Bim and Bmf were efficiently depleted in 3D spheroids.
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