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Another important consideration is the limited number of payload molecules that can be efficiently delivered into target cells.
The intracellular uptake assay proved that DOX from DOX-loaded SCMs could be efficiently delivered into HepG2 cells after 12 h incubation.
Since the material degradation was retarded upon loading with hydrophobic guest molecules, the cargo could be efficiently delivered into live cancer cells and released intracellularly without pronounced premature release under extracellular conditions.
The results demonstrate that with the combination of flap, WPRE sequences, and the promoter derived from spleen focus-forming virus (SFFV), a foreign gene can be efficiently delivered into primary human T lymphocytes and cord blood CD34+ cells.
Overall results indicated that aptameric oligomers complexed with polycations can be efficiently delivered into the cells and display the desired biological effect designing a balanced partial duplex whose stability can allow oligomer release from the polycation under the physiological cellular conditions.
In conclusion, these results indicated that all heterologous genes on the T-DNA could be efficiently delivered into the plant genome as a whole, and could be successfully coexpressed.
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Cellular uptake experiments revealed that these pH-responsive HA-PHis micelles taken up in great amounts by receptor-mediated endocytosis and DOX were efficiently delivered into cytosol.
Epifluorescence microscopy analysis of CNT/DNA conjugates indicates that these conjugates were efficiently delivered into cells and distributed within the cytoplasm, although the question of CNT conjugate penetration into nuclei remains unresolved at this time.
Phytosphingosine-grafted polymeric micelle-like aggregates as small as 20 nm were efficiently delivered into various cancer cell lines including oral, breast and colon carcinoma with the extent which is comparable to the level of targeting carrier system.
CASP3 was efficiently delivered into cells, resulting in effective induction of apoptosis.
Intravenously administrated IHL-305 was efficiently delivered into peritoneal ascites; this drug delivery system prolonged the survival of the mouse with peritoneal metastasis.
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