Sentence examples for be efficiently cloned as from inspiring English sources

While transcripts from several HML-2 loci with one or two mismatches to the primers were eventually cloned as cDNA, it is difficult to decide for other loci whether they were not expressed or could not be efficiently cloned as cDNA for technical reasons.

The results show that, by using this platform, genes encoding membrane proteins can be efficiently cloned for initial expression screening.

Nested PCR products were purified and cloned as above.

These vectors can be efficiently retrofitted onto BACs without rearrangement of the BAC clone.

Secondly the target open reading frames must be efficiently converted into expression templates (preferably without the need for cloning).

Use of DNA (rather than mRNA) for microarray-based deletion cloning strategies has a number of benefits, as the mRNA abundance of many genes is too low to be efficiently and reliably detected.

where, is efficiently obtained as in (12).

Amplicons were cloned as previously described.

PCR amplicons were cloned as described above.

Through 400 PCR reactions, 765,696 clones were efficiently tested with the Yr26-linked marker Xwe173.

From the 14 clones selected previously, 10 scFvs were efficiently expressed and purified before measuring their affinities for BoNT/A.