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As with other recent studies [ 5, 7, 15, 16], our results indicate that short reads from 454 sequencing runs can be effectively assembled and used to readily characterize the gene space of non-model organisms.
Studies using transcriptome sequencing for organisms with complete genome sequencing have confirmed that the short-read products of NGS can be effectively assembled and used for gene discovery and comparison of gene expression profiles.
Consistent with these reports, the results from this research also suggested that short reads from Illumina sequencing can be effectively assembled and used for gene identification and SSR marker development in non-model organisms.
Previously, Illumina sequencing of transcriptomes for organisms with completed genomes confirmed that the relatively short reads produced can be effectively assembled and used for gene discovery and comparison of gene expression profiles [ 21, 22].
Some recent studies have indicated that short reads from 454 GS FLX pyrosequencing may be effectively assembled and used to characterise the gene space in various organisms [ 16, 21, 32, 33].
454 sequencing of transcriptomes for model organisms has confirmed that the relatively short reads produced by this technology can be effectively assembled and used for gene discovery [ 25, 26].
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Our results, along with several other recent studies, indicate that short reads from Illumina sequencing can be effectively assembled to provide a characterisation of the transcribed genes within a non-model species [ 8, 9, 11, 13, 15, 17, 25, 30].
Over the last two years, with the further confirmation that the relatively short reads can be effectively assembled [ 15], especially with the great advantage of paired-end sequencing [ 54], the Illumina transcriptome or whole genome de novo sequencing and assembly have been successfully used for model [ 12, 16, 55- 58]anon-modeldel organisms [ 25- 27, 59].
The novel hinge-binder tethered 1,2,3-triazolylsalicylamide scaffold was effectively assembled by Cu(I -catalyzed azI -catalyzed,3-dipolazide alkynetion (CuAAC).
These results suggested that the transcriptome sequencing data from A. racemosus were effectively assembled, which was further validated by the high proportion of unigenes matched with known proteins.
These results suggested that the transcriptome sequencing data from rubber tree bark were effectively assembled, which was further validated by the high proportion of unigenes matched with known proteins and the high PCR success rate of EST-SSR markers developed from the assembled unigenes.
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