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However, it should be noted that not all positions with an error prone sequence motif seems to be effected by sequencing coverage bias.
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For example, metagenomic gene prediction can be effected by the ability to correctly assemble metagenomic sequence reads into longer contigs, and this process can be subsequently impacted by factors such as sequencing coverage and chimerism (14).
However, Meyers et al. [ 33] estimated that only ~8% of sequences would be affected by this source of bias and in our libraries this effect will have been suppressed by sequencing in two staggered phases, since this increases the number of tags that do not contain 4 base palindromes in at least one of the phases.
The promoter sequence was analysed by sequencing.
The insert sequences were confirmed by sequencing.
Clones were verified by sequencing.
Integrity was confirmed by sequencing.
Plasmids were checked by sequencing.
Constructions were verified by sequencing.
Insertion was confirmed by sequencing.
The construct was checked by sequencing.
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