S. cerevisiae is an especially useful species in which to study the cause of rapid centromere evolution because its point centromeres are easy to sequence and patterns of recombination and mutation within its genome are exceptionally well characterised [ 13, 15, 23, 25, 26].
The team focused on mtDNA, which is often employed in genetic studies because it is easier to sequence and allows analysis of huge population samples.
The DNA barcode fragment of COI was easier to sequence than the other genes, and had high scores for utility but low signal above the genus level.
The DNA barcode was easier to sequence than other genes, and had high scores for utility but low signal above the genus level.
The end product is at least 49 times shorter than the original RNA, so it is easier to sequence.
Therefore, the possibility of a bias for GC-rich sequences due to the small sample size of this dataset could exist, as GC-rich genes with few or small introns were easier to sequence because of their shorter size.
It has therefore already been demonstrated that LGT and recombination events are possible, and it may be easy to transfer sequences from microorganisms infecting eukaryotic cells continuously over such a long period of time.
"The initial, somewhat naive optimism -- that it was going to be easy to be able to translate [genome sequencing] into improving people's health and quality of life -- has met the reality of just how complicated it is, and how biological systems are not linear," explained Marcom.
It's easy to vary a sequence of events — like choosing a restaurant for a weekly date night — without offering a lot of surprise.
