We found that in migratory cells, 84 genes were significantly upregulated and 103 were downregulated compared to the neuroepithelial clusters.
Specifically, DNA-PKcs and PCNA were upregulated in TMC, and XPA was downregulated compared to pre-senescence MSC.
However, our results differed at the protein level compared to a study on cold thyroid nodules where both the expression of CRABP1 mRNA and the protein were downregulated compared to normal surrounding tissue [41].
Figure 2B also shows that when elt-2 expression is ablated by RNAi, clec-67 is downregulated compared to vector control, indicating that ELT-2 regulates the expression of this effector of C. elegans immunity to S. enterica.
RT-PCR showed that the expression of MYH9 in GC9811 cells and SGC7901-NM cells transfected with let-7f-mimics is downregulated compared to those cells transfected with control constructs (Figure 5D).
Western blot analysis confirmed that PLA2G4A protein expression was downregulated compared to POF ovarian tissue).
In 87% (217/250), E-cadherin expression was downregulated compared to normal mucosa (Blechschmidt score [ 26]).
In siPCA3/LNCaP-transfected cells, the expression of AR target genes was downregulated compared to siSCr-transfected cells.
Array data showed that 14 miRNAs were upregulated and 1 miRNA was downregulated compared to normal peritoneal tissue [ 32].
An important finding of the study is that in periodontitis sites following non-surgical therapy the overall expression profile was downregulated compared to healthy controls.
In mice with intermediate core expression (experiment II), 194 genes were differentially expressed (120 were upregulated and 74 were downregulated) compared to the STM liver.
