PCR amplifications were done with specific sets of primers designed from the conserved exonic regions of B. azoricus EST sequences, for which an intron was known to occur in other taxa.
PCR experiments were done with specific primers for external controls (see Additional file 2): 30 cycles of amplification were performed, using different amounts of plasmid DNA from each library (A, I or N) as a template.
To further determine the possibility of NF-κB binding to MMP-2/MMP-9 promoter binding sites, EMSA was done with specific oligonucleotides As shown in Figure 6C, the TGF-β1-stimulated NF-κB DNA binding activities were strongly inhibited by pinocembrin at the concentration of 5 μM.
Motivation to Sue But, now doubt, you will want to quibble that the ordinance in forbidding these activities requires not only that the activity take place, but that it be done with the specific intent of chasing the tenant away from its tenancy.
However, the difference in sPLA2/SP-A ratio between cases and controls is not statistically significant, possibly due to our small population size: claryfing the role of SP-A is worth to be done with a specific study.
A dedicated research can be done with selecting specific amino acid types, secondary structures, SCOP class classification (30), protein length, as well as number of PTMs observed in PDB chain.
Each project may have several and maybe small tasks that need to be done with the specific skill or experience that your team members may not posses.
When I talked to product manager Josh Williams ahead of the launch of Facebook's new location-discovery service Nearby, and to CTO Cory Ondrejka at a Facebook reporters event, both said there were interesting things to be done with content-specific feeds.
The evaluation of breast implants, which are either placed for breast augmentation or for breast reconstruction after surgery for breast cancer, can be done with MR. This demands specific sequences that are aimed at the visualization of silicone and provide concurrent suppression of the water signal [ 115– 115].
Further study needs to be done with large numbers of patients with specific pain site(s).
Transfer of behavior-based pain evaluation systems developed for clinical science to ridden horses must be done with caution, since research methods specific for evaluation of pain in equitation science have not been validated.
