Radiolabeling was done using non-site-specific and site-specific conjugation approaches with N-succinimidyl 4-[18F]fluorobenzoate ([18F]-SFB) and the bifunctional chelator 1,4,7-triazacyclononane-triacetic acid maleimide (NOTA-maleimide) and copper-64 ([64Cu]), respectively.
Because our assays were done using cell lysate, we used the straightforward technique of body-radiolabeling input reporter mRNAs and then incubating them in rabbit reticulocyte lysate over the time course and temperature of the translation experiments.
The radiolabelling and purification of the PSMA ligand was done using an automated module.
The radiolabelling could still be done by using [18F]FDR as a prosthetic group, making the oxime radiolabelling approach very appealing to be used with tetrazines due to the mild reaction conditions of the glycosylation.
Expression analyses were done in synovial fibroblasts from RA patients by quantitative real-time polymerase chain reaction, and cytochrome b5 expression was detected by immunohistochemistry. Real-life androgen production after steroid conversion was measured using radiolabeled substrates.
Radiolabelling of the hu3S193 antibody constructs with indium-111 and lutetium-177 was done by using the bifunctional metal ion chelate C-functionalized trans-cyclohexyl diethylenetriaminepentaacetic acid (CHX-A″ DTPA) [34, 35].
Genomic DNA digested with BamHI was analysed by Southern hybridization using radiolabelled hpt gene probe [ 21].
Another strategy using radiolabeling by isotopic exchange is based on boron derivatives.
Radiolabelling for the pretherapy dose was done at the local Nuclear Medicine department, using the Iodogen method.
SK1 assay was performed using radiolabelling as previously described [ 21].
